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KMID : 1059519970410070343
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Journal of the Korean Chemical Society
1997 Volume.41 No. 7 p.343 ~ p.350
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Chicken Liver Tissue-Based Amperometric Biosensor for the Determination of Hydrogen Peroxide
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Yoon Kil-Joong
Pyun Sang-Yong
Kwon Hyo-Shik
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Abstract
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The response characteristics of a new biosensor, which was developed by co-immobilisation of chicken liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide, was evaluated. In the range of electrode potential observed (-O.5∼ +0.05 V vs. Ag/AgCl), the bioelectrode showed the response time (t95%) as low as 13 set and the detection limit of 5.1x10-5 M, and exhibited a good selectivity in the case of the addition of the possible interferents tested. Also it offered a high biocatalytic stability and opened up the possibility for the construction of a mediatorless biosensor.
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